Peptide Dosing Advice: Why Most Dosage Charts Get It Wrong
What Determines Your Peptide Dosage?
Good peptide dosing advice starts with four things, in order. The compound, since doses range from micrograms to milligrams. Its half-life (how long it stays in the body), which varies widely with how the peptide is modified. How you mixed it, which sets what each draw contains. And whether the vial matches its label, which decides if the rest matters.
Most dosing advice skips straight to a number. That number is the last step, not the first. A chart with no arithmetic behind it is asking you to trust a stranger's guesswork.
This content is for educational purposes only. These compounds are intended for research use. Nothing here is medical advice. Any decision about a protocol should involve a qualified clinician.
What Does This Peptide Dosage Chart Show You?
This chart covers twelve compounds. For each, it gives what a named primary source reports: an approved label, a dose a study used, or what FDA found sellers offering. None of it is a recommended dose. Amounts are in mcg (micrograms) or mg (milligrams).
| Compound | What the named source reports | Route in that source |
|---|---|---|
| BPC-157 | FDA's 2026 review found one compounder selling 500 mcg capsules, with directions to take one twice daily. Human studies it reviewed used other doses, such as an 80 mg enema once daily for two weeks | By mouth (compounder); rectal enema (study) |
| TB-500 | FDA's 2026 review found no clinical studies or human exposure data, so there is no human dose on record | Not studied in people |
| CJC-1295 | In the studies FDA reviewed in 2024, 63 healthy adults got 30 to 250 mcg per kg (per kilogram of body weight) of the DAC form (a long-acting version), most as a single injection | Under the skin (subcutaneous) |
| Ipamorelin | A trial after bowel surgery gave 0.03 mg per kg by drip into a vein, twice daily for up to 7 days (Beck et al. 2014) | Into a vein (intravenous) |
| MOTS-c | FDA's 2026 review found no clinical studies or human exposure data, so there is no human dose on record | Not studied in people |
| KPV | FDA's 2026 review found no clinical studies or human exposure data, so there is no human dose on record | Not studied in people |
| Thymosin Alpha-1 | Its label as Zadaxin in Indonesia, as FDA's 2024 review reports it: 1.6 mg twice weekly for chronic hepatitis B | Under the skin (subcutaneous) |
| Semax | Registered in Russia as 0.1% and 1% nasal drops. One article in FDA's 2026 review describes 1% drops, 2 to 4 drops three times a day for 10 days | In the nose (intranasal) |
| DSIP | Sleep studies in FDA's 2026 review gave emideltide (DSIP) at 25 or 30 nmol per kg (nanomoles, a count of molecules) into a vein in the evening | Into a vein (intravenous) |
| AOD-9604 | In the OPTIONS trial in FDA's 2024 review, 502 adults with obesity were randomised to 0.25, 0.5 or 1 mg by mouth once daily, or placebo, for 24 weeks | By mouth |
| Kisspeptin-10 | Research studies in FDA's 2024 review gave boluses (single quick doses) into a vein of up to 13 mcg per kg, and single injections under the skin of up to 42 mcg per kg | Into a vein or under the skin |
| Dihexa | PubMed listed no study of dihexa in people when searched in September 2026, so there is no human dose on record | Not studied in people |
Read this as a record of what each source reports, not a prescription. Where no human study exists, there is no human dose to report. Our dosing articles go further: BPC-157 dosing, TB-500 dosage, MOTS-c dosage, ipamorelin dosage and CJC-1295 with and without DAC. Others cover thymosin alpha-1 dosage, tesamorelin, retatrutide titration and AOD-9604 dosage.
How Do You Work Out a Peptide Dosage From Your Vial?
Our peptide dosing calculator does this arithmetic for you. It asks for the vial size and the water you added, so check both before you trust the answer.
Your dose is not a property of the vial. It is a property of how much water you added. Get that wrong, and every number after it is wrong too.
Do the maths in two lines. Divide the milligrams (mg) in the vial by the millilitres (ml) of bacteriostatic water (the sterile mixing water) you added. That gives milligrams per millilitre. Then divide your target dose by that figure to get the volume to draw.
Add 2ml to a 5mg vial and each millilitre holds 2.5mg. Add 3ml to the same vial and each millilitre holds about 1.67mg. Same powder. Different dose in the same syringe marking.
Our reconstitution chart lays out the common combinations. The microgram to syringe units chart converts your result into the marks on an insulin syringe. The full reconstitution walkthrough covers water choice, storage and stability.
What's on This Peptide Dosage Cheat Sheet?
Five rules are worth remembering before you dose anything.
- Half-life varies with how a peptide is modified. In one review of animal studies, it ran from 0.1 to 33.57 hours.
- Micrograms and milligrams are a thousand apart.
- Reconstitution volume sets your dose. Write it on the vial.
- A dose is only as real as the vial. Purity and content are different measurements.
- If a chart cites no source, it is someone's habit, not evidence.
Why Does the Vial Matter More Than the Chart?
Because it assumes the vial matches the label. A dosing schedule built on 5mg of BPC-157 does nothing useful if the vial holds 3mg of something else. Purity and net peptide content are measured differently.
Purity describes how clean the peptide is, relative to itself. It says nothing about how much peptide is in there, against water, salts and counterions (charged molecules that cling to the peptide).
That gap is real, not theoretical. No production method yields only the target peptide. Chemical synthesis leaves truncated fragments (cut-short chains) and deletion sequences (chains missing a building block) behind, from steps that did not fully complete. That is why preparative liquid chromatography (a lab method that sorts molecules to purify them) is a standard purification step (De Luca et al. 2021). It separates the peptide you want from these production leftovers.
What's left over matters in your body, not just on paper. A 2025 case study, by researchers at EpiVax with FDA co-authors, tested impurities found in generic teriparatide, a peptide drug. It used computer models and lab tests to check whether each impurity could trigger an unwanted immune response (Mattei 2025). FDA's 2026 review of BPC-157 makes a similar point. FDA says BPC-157 may pose a risk of an unwanted immune response, potentially amplified by clumps of peptide (aggregates) and peptide-related impurities.
So before you trust any number, ask for three things. A batch-specific certificate showing HPLC (the standard lab purity test) purity, with the chromatogram (the trace chart the test prints). Our sources standard ideally looks for 98 to 99% or higher. A mass spectrometry identity report (a test that confirms the molecule by its weight). And a named lab you can check for ISO/IEC 17025 accreditation (the international standard for testing labs). Our guide to reading a peptide COA (certificate of analysis) covers what a real one contains. Independent purity testing covers who does the testing.
Why Does Peptide Half-Life Vary So Widely?
This is the part most charts leave out.
Chemical modification changes how long a peptide lasts in the blood. A 2022 systematic review (a study that pools earlier studies) measured half-life extension across conjugation methods (ways of bonding on another molecule). Its studies gave the peptides into a vein in rodents (mice, rats) and monkeys, and it left out injections under the skin. Half-lives ranged from roughly 0.1 hours up to 33.57 hours, depending on the modification (Wijesinghe 2022).
Those are animal figures, not a human dosing schedule. Fatty-acid conjugated and PEGylated versions need that modification confirmed by mass spectrometry, not assumed from the name on the vial. PEGylated means bonded to a polymer called PEG. If you are copying a frequency from a forum post, you are assuming their vial and yours were modified the same way.
Where to buy peptide dosing
If you're researching how peptide dosing actually gets validated, I've linked a trusted source below. It supports the channel.
See the sources that passed →How Do You Verify a Peptide Before You Dose It?
Treat verification as step one, not an afterthought.
Confirm the compound is what the label says, at the purity claimed, from a lab you can check independently. Two people running an identical schedule can get different outcomes. The reason often has nothing to do with pharmacokinetics (how your body processes the compound). One person is dosing a verified compound. The other is not. Our recommended sources page lists vendors who publish batch-level documentation, and the peptide purity crisis article covers the structural side of the problem.
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Frequently Asked Questions
What purity percentage should a peptide COA show?
Can you tell a fake or underdosed peptide just by looking at it?
What is the difference between purity and net peptide content?
How do I know if a testing lab on a COA is legitimate?
Why does sourcing matter more than dosing calculations?
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Disclaimer: This content is for educational purposes only. These compounds are intended for research use. Nothing here is medical advice. Always work with a qualified clinician before making changes to your health protocol.