GHK-Cu dosing calculator
1 mg = 1000 mcg.
0.060 mL · 33,333 mcg/mL
- Doses per vial
- 50
- Per unit
- 333.3 mcg
- One vial lasts
- 50 days at 1/day
Your draw
www.undergroundbiohacking.com · For tracking and calculation purposes only. Not medical advice.
For tracking and calculation purposes only. Not medical advice. Based on a standard U-100 insulin syringe.
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How the units are worked out
Your vial holds a fixed amount of peptide. The water you add sets the concentration (how much peptide sits in each millilitre). The concentration then decides how much liquid carries your dose.
Three steps, in order:
- Concentration (mcg/mL) = peptide in the vial (mcg) divided by water added (mL).
- Volume (mL) = your dose (mcg) divided by the concentration (mcg/mL).
- Units to draw = volume (mL) multiplied by 100.
That last step is the syringe, not the peptide. A U-100 insulin syringe is marked so 100 units hold 1 mL, so 0.1 mL reads as 10 units.
A worked example
These are the numbers in the calculator above, worked through: 100 mg + 3 mL = 33,333 mcg/mL. 2000 mcg = 0.06 mL = 6 units on a 0.3 mL syringe.
Change any number in the tool and this line changes with it. The link in your address bar carries the numbers, so you can save it or send it to yourself.
Water and units at a glance
The same dose of GHK-Cu at different mixes. Every row opens the calculator on that mix.
| Vial | Water | Concentration | Units to draw |
|---|---|---|---|
| 100 mg | 1 mL | 100,000 mcg/mL | 2 units |
| 100 mg | 2 mL | 50,000 mcg/mL | 4 units |
| 100 mg | 3 mL | 33,333 mcg/mL | 6 units |
| 100 mg | 5 mL | 20,000 mcg/mL | 10 units |
Pre-filled numbers are the most-used setups reported by users of public calculators (WPA dosing data, Aug 2026). Not a recommendation.
Mixing and storage
Run the water down the inside wall of the vial and let the powder dissolve on its own. Never shake it. Label the vial with the compound, the total mass, the water you added, the concentration and the date. A sealed freeze-dried vial keeps 12 to 24 months in the fridge at 2 to 8°C. Once mixed with preserved water, such as bacteriostatic water, that drops to roughly 28 days refrigerated. Keep it at the back of the fridge, away from light and away from the door's temperature swings.
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Questions
Does GHK-Cu actually reduce wrinkles?
Is GHK-Cu better as a cream or as an injection?
Does GHK-Cu regrow hair?
Is GHK-Cu safe?
Will GHK-Cu heal a wound or a scar faster?
What should I ask a doctor before using GHK-Cu?
References
- 01Miller TR, et al. (2006). Effects of topical copper tripeptide complex on CO2 laser-resurfaced skin. Archives of facial plastic surgery. PMID: 16847171. DOI: 10.1001/archfaci.8.4.252.
- 02Deng M, et al. (2023). Glycyl-l-histidyl-l-lysine-Cu2+ rescues cigarette smoking-induced skeletal muscle dysfunction via a sirtuin 1-dependent pathway. Journal of cachexia, sarcopenia and muscle. PMID: 36905132. DOI: 10.1002/jcsm.13213.
- 03Trookman NS, et al. (2009). Clinical assessment of a combination lip treatment to restore moisturization and fullness. The Journal of clinical and aesthetic dermatology. PMID: 20725584.
- 04Maquart FX, et al. (1993). In vivo stimulation of connective tissue accumulation by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu2+ in rat experimental wounds. The Journal of clinical investigation. PMID: 8227353. DOI: 10.1172/JCI116842.
- 05Cangul IT, et al. (2006). Evaluation of the effects of topical tripeptide-copper complex and zinc oxide on open-wound healing in rabbits. Veterinary dermatology. PMID: 17083573. DOI: 10.1111/j.1365-3164.2006.00551.x.
- 06Canapp SO, et al. (2003). The effect of topical tripeptide-copper complex on healing of ischemic open wounds. Veterinary surgery : VS. PMID: 14648529. DOI: 10.1111/j.1532-950x.2003.00515.x.
- 07Wegrowski Y, et al. (1992). Stimulation of sulfated glycosaminoglycan synthesis by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu2+. Life sciences. PMID: 1522753. DOI: 10.1016/0024-3205(92)90504-i.
- 08Hostynek JJ, et al. (2010). Human skin retention and penetration of a copper tripeptide in vitro as function of skin layer towards anti-inflammatory therapy. Inflammation research : official journal of the European Histamine Research Society. PMID: 20703511. DOI: 10.1007/s00011-010-0214-4.
- 09Simeon A, et al. (1999). Expression and activation of matrix metalloproteinases in wounds: modulation by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu2+. The Journal of investigative dermatology. PMID: 10383745. DOI: 10.1046/j.1523-1747.1999.00606.x.
- 10Bishop JB, et al. (1992). A prospective randomized evaluator-blinded trial of two potential wound healing agents for the treatment of venous stasis ulcers. Journal of vascular surgery. PMID: 1495150. DOI: 10.1067/mva.1992.37086.
By Chris Hallewell, Founder, Underground Biohacking
Last reviewed
The numbers here describe your own vial. They are not medical advice and not a dose suggestion. Confirm your protocol with a qualified clinician.
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