Underground Biohacking

GHK-Cu dosing calculator

CompoundGHK-Cu
mg
mL

1 mg = 1000 mcg.

Your syringe
Draw this much
0.0units

0.060 mL · 33,333 mcg/mL

510152025301020306.0
0 units0.3 mL barrel, U-100 scale30 units
Doses per vial
50
Per unit
333.3 mcg
One vial lasts
50 days
Reference only · Not medical advice

Your draw

Vial100 mg
Water3 mL
Concentration33,333 mcg/mL
Dose2000 mcg
Draw6 units
Syringe0.3 mL, U-100

www.undergroundbiohacking.com · For tracking and calculation purposes only. Not medical advice.

For tracking and calculation purposes only. Not medical advice. Based on a standard U-100 insulin syringe.

How the units are worked out

Your vial holds a fixed amount of peptide. The water you add sets the concentration (how much peptide sits in each millilitre). The concentration then decides how much liquid carries your dose.

Three steps, in order:

  1. Concentration (mcg/mL) = peptide in the vial (mcg) divided by water added (mL).
  2. Volume (mL) = your dose (mcg) divided by the concentration (mcg/mL).
  3. Units to draw = volume (mL) multiplied by 100.

That last step is the syringe, not the peptide. A U-100 insulin syringe is marked so 100 units hold 1 mL, so 0.1 mL reads as 10 units.

A worked example

These are the numbers in the calculator above, worked through: 100 mg + 3 mL = 33,333 mcg/mL. 2000 mcg = 0.06 mL = 6 units on a 0.3 mL syringe.

Change any number in the tool and this line changes with it. The link in your address bar carries the numbers, so you can save it or send it to yourself.

Water and units at a glance

The same dose of GHK-Cu at different mixes. Every row opens the calculator on that mix.

VialWaterConcentrationUnits to draw
100 mg1 mL100,000 mcg/mL2 units
100 mg2 mL50,000 mcg/mL4 units
100 mg3 mL33,333 mcg/mL6 units
100 mg5 mL20,000 mcg/mL10 units

Pre-filled numbers are the most-used setups reported by users of public calculators (WPA dosing data, Aug 2026). Not a recommendation.

Mixing and storage

Run the water down the inside wall of the vial and let the powder dissolve on its own. Never shake it. Label the vial with the compound, the total mass, the water you added, the concentration and the date. A sealed freeze-dried vial keeps 12 to 24 months in the fridge at 2 to 8°C. Once mixed with preserved water, such as bacteriostatic water, that drops to roughly 28 days refrigerated. Keep it at the back of the fridge, away from light and away from the door's temperature swings.

Other compounds

Questions

Does GHK-Cu actually reduce wrinkles?
No published trial has shown that convincingly. The randomised human trial we found used GHK-Cu skin care after CO2 laser resurfacing. Blinded evaluators scored wrinkles and overall skin quality, and found no difference between the group that used it and the group that did not. The patients themselves rated their skin as better. That gap, between what people feel and what independent raters can measure, is the whole story with this peptide.
Is GHK-Cu better as a cream or as an injection?
Creams are where the human testing has actually happened, and even there the results are weak. Injections have no published human efficacy trial that we could find. FDA specifically flags injectable GHK-Cu for a possible immune reaction and says human safety data are limited. GHK-Cu also loves water, so it does not cross the oily outer skin layer easily. That is a formulation problem to solve, not a reason to inject it.
Does GHK-Cu regrow hair?
There is no human trial of GHK-Cu for hair loss that we could find. The hair claims trace back to laboratory and animal work, and often to products where GHK-Cu is one ingredient sitting alongside several growth factors. In those cases you cannot tell what the peptide itself contributed. Review articles repeat the claim, but a review is not new evidence. Treat hair regrowth claims for GHK-Cu as untested in people.
Is GHK-Cu safe?
For injections, honestly, nobody knows. No trial has been published that set out to measure the side effects of injected GHK-Cu in people. FDA says the human safety data are limited and flags a possible immune reaction. Topical use has a longer track record and looks mild. One four week study of a lip product containing a GHK derivative reported no adverse events in 32 women. That is one small, short study of one product.
Will GHK-Cu heal a wound or a scar faster?
In animals, fairly consistently yes. Rabbit and rat wound studies found faster closure with topical copper tripeptide than with control treatments. In people, the one sizeable trial tested a copper tripeptide cream on venous leg ulcers and it did no better than placebo. Silver sulfadiazine beat both. So the animal signal is real and the human confirmation has not arrived. Do not use it in place of proper wound care.
What should I ask a doctor before using GHK-Cu?
Ask which route they are proposing and why. Injectable GHK-Cu is not an approved medicine, and FDA lists it among peptides with limited human safety data. Ask where the substance was made and how its purity was tested. Ask what they expect it to do that a proven treatment cannot. If you have a wound, a scar or hair loss, ask what the evidence backed option is first.

References

  1. 01Miller TR, et al. (2006). Effects of topical copper tripeptide complex on CO2 laser-resurfaced skin. Archives of facial plastic surgery. PMID: 16847171. DOI: 10.1001/archfaci.8.4.252.
  2. 02Deng M, et al. (2023). Glycyl-l-histidyl-l-lysine-Cu2+ rescues cigarette smoking-induced skeletal muscle dysfunction via a sirtuin 1-dependent pathway. Journal of cachexia, sarcopenia and muscle. PMID: 36905132. DOI: 10.1002/jcsm.13213.
  3. 03Trookman NS, et al. (2009). Clinical assessment of a combination lip treatment to restore moisturization and fullness. The Journal of clinical and aesthetic dermatology. PMID: 20725584.
  4. 04Maquart FX, et al. (1993). In vivo stimulation of connective tissue accumulation by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu2+ in rat experimental wounds. The Journal of clinical investigation. PMID: 8227353. DOI: 10.1172/JCI116842.
  5. 05Cangul IT, et al. (2006). Evaluation of the effects of topical tripeptide-copper complex and zinc oxide on open-wound healing in rabbits. Veterinary dermatology. PMID: 17083573. DOI: 10.1111/j.1365-3164.2006.00551.x.
  6. 06Canapp SO, et al. (2003). The effect of topical tripeptide-copper complex on healing of ischemic open wounds. Veterinary surgery : VS. PMID: 14648529. DOI: 10.1111/j.1532-950x.2003.00515.x.
  7. 07Wegrowski Y, et al. (1992). Stimulation of sulfated glycosaminoglycan synthesis by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu2+. Life sciences. PMID: 1522753. DOI: 10.1016/0024-3205(92)90504-i.
  8. 08Hostynek JJ, et al. (2010). Human skin retention and penetration of a copper tripeptide in vitro as function of skin layer towards anti-inflammatory therapy. Inflammation research : official journal of the European Histamine Research Society. PMID: 20703511. DOI: 10.1007/s00011-010-0214-4.
  9. 09Simeon A, et al. (1999). Expression and activation of matrix metalloproteinases in wounds: modulation by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu2+. The Journal of investigative dermatology. PMID: 10383745. DOI: 10.1046/j.1523-1747.1999.00606.x.
  10. 10Bishop JB, et al. (1992). A prospective randomized evaluator-blinded trial of two potential wound healing agents for the treatment of venous stasis ulcers. Journal of vascular surgery. PMID: 1495150. DOI: 10.1067/mva.1992.37086.
Chris Hallewell, founder of Underground Biohacking

By , Founder, Underground Biohacking

Last reviewed

The numbers here describe your own vial. They are not medical advice and not a dose suggestion. Confirm your protocol with a qualified clinician.

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