Underground Biohacking

Kisspeptin-10 dosing calculator

CompoundKisspeptin-10
mg
mL

1 mg = 1000 mcg.

Your syringe
Draw this much

Enter a vial size, water volume and dose to see where to pull.

51015202530102030
0 units0.3 mL barrel, U-100 scale30 units

For tracking and calculation purposes only. Not medical advice. Based on a standard U-100 insulin syringe.

This calculator has no pre-filled dose for Kisspeptin-10. We pre-fill a dose only from an approved label or a human trial arm that fits this tool, and none does.

How the units are worked out

Your vial holds a fixed amount of peptide. The water you add sets the concentration (how much peptide sits in each millilitre). The concentration then decides how much liquid carries your dose.

Three steps, in order:

  1. Concentration (mcg/mL) = peptide in the vial (mcg) divided by water added (mL).
  2. Volume (mL) = your dose (mcg) divided by the concentration (mcg/mL).
  3. Units to draw = volume (mL) multiplied by 100.

That last step is the syringe, not the peptide. A U-100 insulin syringe is marked so 100 units hold 1 mL, so 0.1 mL reads as 10 units.

A worked example

Enter your dose in the calculator above and this line shows the numbers worked through.

Water and units at a glance

Kisspeptin-10 at different mixes. Every row opens the calculator on that mix.

VialWaterConcentration
10 mg1 mL10,000 mcg/mL
10 mg2 mL5,000 mcg/mL
10 mg3 mL3,333 mcg/mL
10 mg5 mL2,000 mcg/mL

Units appear once you enter a dose.

The pre-filled vial size is the most-used size in WPA dosing data (Aug 2026), or our vendors' most common size where WPA data is thin. The water is the most-used amount in WPA data. There is no pre-filled dose. Not a recommendation.

Mixing and storage

Run the water down the inside wall of the vial and let the powder dissolve on its own. Never shake it. Label the vial with the compound, the total mass, the water you added, the concentration and the date. A sealed freeze-dried vial keeps 12 to 24 months in the fridge at 2 to 8°C. Once mixed with preserved water, such as bacteriostatic water, that drops to roughly 28 days refrigerated. Keep it at the back of the fridge, away from light and away from the door's temperature swings.

Other compounds

Questions

Does kisspeptin-10 raise testosterone?
In men, yes, in the short term. In a hospital study, kisspeptin-10 raised LH quickly, and a continuous infusion raised testosterone as well. That is the whole point of the peptide. It sits above the reproductive axis and switches it on. But those were supervised infusions lasting hours. Nobody has shown that repeated self-injection gives a lasting testosterone gain. One kisspeptin analogue did the opposite when it was given non stop.
Does kisspeptin-10 improve libido?
The sexual desire studies did not use kisspeptin-10. Imperial College London ran randomised trials in men and in women with hypoactive sexual desire disorder. Both used kisspeptin-54, the longer form. In men, kisspeptin-54 changed sexual brain activity and increased physical arousal. In women it changed how the brain processed erotic and attraction cues. Whether the ten amino acid version does the same thing in people has not been tested.
Is kisspeptin-10 the same thing as kisspeptin-54?
No. They come from the same gene and act on the same receptor, but they are different peptides. Kisspeptin-54 is the full chain your body makes. Kisspeptin-10 is the last ten amino acids of that chain. In lab cells the short tail is as potent as the whole peptide. In people the two have been tested in different studies, for different questions, so results from one do not automatically carry over.
Does it work the same way in women and in men?
No. One study gave kisspeptin-10 to both men and women. The men showed a clear rise in LH and FSH. The women responded near ovulation, but showed no gonadotropin rise at all during the early follicular phase. So where a woman sits in her cycle changes the answer completely. That is real biology, not a technique problem, and it makes the effect in women much harder to predict.
Is kisspeptin-10 legal?
It is not an approved medicine. The FDA has placed it in category 2 of its bulk drug substances list under section 503A. That means the agency has flagged potential significant safety risks, and says it lacks the information to know whether kisspeptin-10 would harm people. Nothing about that position has changed.
What should I ask a doctor about it?
Ask what your LH, FSH, testosterone or oestradiol actually look like now, and what is driving the symptom you want fixed. Ask whether an approved treatment already exists for that problem. Ask what a hormone panel would show before and after anything you take. If low desire is the issue, ask about the causes a clinic can treat today. The trials that showed a sexual effect used a different peptide, and they are not a treatment yet.

References

  1. 01Ohtaki T, et al. (2001). Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor. Nature. PMID: 11385580. DOI: 10.1038/35079135.
  2. 02Hori A, et al. (2001). Metastin suppresses the motility and growth of CHO cells transfected with its receptor. Biochemical and biophysical research communications. PMID: 11527393. DOI: 10.1006/bbrc.2001.5470.
  3. 03Dhillo WS, et al. (2005). Kisspeptin-54 stimulates the hypothalamic-pituitary gonadal axis in human males. The Journal of clinical endocrinology and metabolism. PMID: 16174713. DOI: 10.1210/jc.2005-1468.
  4. 04George JT, et al. (2011). Kisspeptin-10 is a potent stimulator of LH and increases pulse frequency in men. The Journal of clinical endocrinology and metabolism. PMID: 21632807. DOI: 10.1210/jc.2011-0089.
  5. 05Jayasena CN, et al. (2011). The effects of kisspeptin-10 on reproductive hormone release show sexual dimorphism in humans. The Journal of clinical endocrinology and metabolism. PMID: 21976724. DOI: 10.1210/jc.2011-1408.
  6. 06MacLean DB, et al. (2014). Sustained exposure to the investigational Kisspeptin analog, TAK-448, down-regulates testosterone into the castration range in healthy males and in patients with prostate cancer: results from two phase 1 studies. The Journal of clinical endocrinology and metabolism. PMID: 24762108. DOI: 10.1210/jc.2013-4236.
  7. 07Comninos AN, et al. (2017). Kisspeptin modulates sexual and emotional brain processing in humans. The Journal of clinical investigation. PMID: 28112678. DOI: 10.1172/JCI89519.
  8. 08Millar RP, et al. (2017). Hypothalamic-Pituitary-Ovarian Axis Reactivation by Kisspeptin-10 in Hyperprolactinemic Women With Chronic Amenorrhea. Journal of the Endocrine Society. PMID: 29264460. DOI: 10.1210/js.2017-00328.
  9. 09Thurston L, et al. (2022). Effects of Kisspeptin Administration in Women With Hypoactive Sexual Desire Disorder: A Randomized Clinical Trial. JAMA network open. PMID: 36287566. DOI: 10.1001/jamanetworkopen.2022.36131.
  10. 10Mills EG, et al. (2023). Effects of Kisspeptin on Sexual Brain Processing and Penile Tumescence in Men With Hypoactive Sexual Desire Disorder: A Randomized Clinical Trial. JAMA network open. PMID: 36735255. DOI: 10.1001/jamanetworkopen.2022.54313.
Chris Hallewell, founder of Underground Biohacking

By , Founder, Underground Biohacking

Last reviewed

The numbers here describe your own vial. They are not medical advice and not a dose suggestion. Confirm your protocol with a qualified clinician.

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