AOD-9604 dosing calculator

CompoundAOD-9604

Vial

Water

Your dose

1 mg = 1000 mcg.

Your syringe

Draw this much

0.0units

0.200 mL · 2,500 mcg/mL

0 units0.3 mL barrel, U-100 scale30 units

Doses per vial: 10
Per unit: 25 mcg
One vial lasts: 10 days at 1/day

Reference only · Not medical advice

Your draw

Vial5 mg

Water2 mL

Concentration2,500 mcg/mL

Dose500 mcg

Draw20 units

Syringe0.3 mL, U-100

www.undergroundbiohacking.com · For tracking and calculation purposes only. Not medical advice.

For tracking and calculation purposes only. Not medical advice. Based on a standard U-100 insulin syringe.

How the units are worked out

Your vial holds a fixed amount of peptide. The water you add sets the concentration (how much peptide sits in each millilitre). The concentration then decides how much liquid carries your dose.

Three steps, in order:

Concentration (mcg/mL) = peptide in the vial (mcg) divided by water added (mL).
Volume (mL) = your dose (mcg) divided by the concentration (mcg/mL).
Units to draw = volume (mL) multiplied by 100.

That last step is the syringe, not the peptide. A U-100 insulin syringe is marked so 100 units hold 1 mL, so 0.1 mL reads as 10 units.

A worked example

These are the numbers in the calculator above, worked through: 5 mg + 2 mL = 2,500 mcg/mL. 500 mcg = 0.20 mL = 20 units on a 0.3 mL syringe.

Change any number in the tool and this line changes with it. The link in your address bar carries the numbers, so you can save it or send it to yourself.

Water and units at a glance

The same dose of AOD-9604 at different mixes. Every row opens the calculator on that mix.

Vial	Water	Concentration	Units to draw
5 mg	1 mL	5,000 mcg/mL	10 units
5 mg	2 mL	2,500 mcg/mL	20 units
5 mg	3 mL	1,667 mcg/mL	30 units
5 mg	5 mL	1,000 mcg/mL	50 units

Pre-filled numbers are the most-used setups reported by users of public calculators (WPA dosing data, Aug 2026). Not a recommendation.

Mixing and storage

Run the water down the inside wall of the vial and let the powder dissolve on its own. Never shake it. Label the vial with the compound, the total mass, the water you added, the concentration and the date. A sealed freeze-dried vial keeps 12 to 24 months in the fridge at 2 to 8°C. Once mixed with preserved water, such as bacteriostatic water, that drops to roughly 28 days refrigerated. Keep it at the back of the fridge, away from light and away from the door's temperature swings.

Other compounds

Questions

Does AOD-9604 burn fat in humans?

Nobody has published a human trial that answers that. The fat loss findings come from obese mice and rats. In those animals the peptide slowed weight gain and raised fat burning. Animals are not people, and obesity drugs fail at exactly this step all the time. Human trials were run in the early 2000s, but their results never appeared in the medical literature. So the honest answer is that the human effect is unknown.

What did the human obesity trials actually find?

That is the frustrating part. A drug profile from 2004 confirms that phase 2a trials were underway by February 2002. Searching PubMed today returns no published report of any AOD-9604 trial result in people. The drug never reached approval in any country. Reviews written in 2026 still list it as an unapproved peptide with no clinical trial evidence behind it. Trials that are never published cannot be checked, so they count for nothing.

Is AOD-9604 safer than growth hormone itself?

That was the whole idea, and the animal data support it in part. Whole growth hormone pushes blood sugar up and can blunt insulin. In obese mice, AOD-9604 did neither. In obese rats, insulin sensitivity held steady on a euglycemic clamp, a test that measures how well insulin is working. In cells the fragment also failed to bind the growth hormone receptor. None of this has been confirmed in people.

Is AOD-9604 approved, and can a doctor prescribe it?

No regulator has approved AOD-9604 as a medicine. In the United States, the FDA lists it among bulk drug substances that may present significant safety risks. Its nomination for use in pharmacy compounding was withdrawn by the nominators. FDA has said it lacks the safety information needed to judge whether the drug would harm people. Advisory committee votes are advice, not law, and they do not change that position.

What are the risks of taking it?

The honest answer is that they are poorly described. There is no published human safety record for AOD-9604 itself. Doctors who treat users of growth hormone axis peptides report hormone and metabolic disturbance, fluid retention, muscle and joint pain, and injection site reactions. Product quality is a separate risk. Material bought outside the medical system may not contain what the label claims.

Does AOD-9604 help joints or cartilage?

The joint claim rests largely on one rabbit study. Researchers injected the knees of rabbits with chemically damaged joints. Cartilage looked better than in untreated animals, and the mix with hyaluronic acid worked best of all. That is a real finding in rabbits. It is not evidence in humans. A 2026 orthopaedic review says plainly that clinical trials of these peptides are still missing. Ask an orthopaedic doctor before you consider it.